High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface.

@article{Binder2010HighthroughputSO,
  title={High-throughput sorting of an Anticalin library via EspP-mediated functional display on the Escherichia coli cell surface.},
  author={Uli Binder and Gabriele Matschiner and Ina Theobald and Arne Skerra},
  journal={Journal of molecular biology},
  year={2010},
  volume={400 4},
  pages={783-802}
}
We demonstrate that small engineered single-chain binding proteins based on the lipocalin scaffold, so-called Anticalins, can be functionally displayed on the Gram-negative bacterial cell envelope. To this end, the beta-domains of five different bacterial autotransporters (the IgA protease from Neisseria gonorrhoeae, the esterase EstA from Pseudomonas aeruginosa, the YpjA autotransporter from E. coli K12, the AIDA-I adhesin from enteropathogenic E. coli O127:H27 strain 2787 and the protease… CONTINUE READING
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