High-throughput single-molecule fluorescence spectroscopy using parallel detection.


Solution-based single-molecule fluorescence spectroscopy is a powerful new experimental approach with applications in all fields of natural sciences. The basic concept of this technique is to excite and collect light from a very small volume (typically femtoliter) and work in a concentration regime resulting in rare burst-like events corresponding to the transit of a single-molecule. Those events are accumulated over time to achieve proper statistical accuracy. Therefore the advantage of extreme sensitivity is somewhat counterbalanced by a very long acquisition time. One way to speed up data acquisition is parallelization. Here we will discuss a general approach to address this issue, using a multispot excitation and detection geometry that can accommodate different types of novel highly-parallel detector arrays. We will illustrate the potential of this approach with fluorescence correlation spectroscopy (FCS) and single-molecule fluorescence measurements obtained with different novel multipixel single-photon counting detectors.

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@article{Michalet2010HighthroughputSF, title={High-throughput single-molecule fluorescence spectroscopy using parallel detection.}, author={Xavier Michalet and Ryan A. Colyer and Giuseppe Scalia and Teawoong Kim and Moran Levi and Daniel B. Aharoni and Adrian Cheng and Fabrizio Guerrieri and Katsushi Arisaka and Jacques Millaud and Ivan Rech and Daniele Resnati and Stefano Marangoni and A Gulinatti and Mariacristina Ghioni and Simone Tisa and Franco Zappa and Sergio Cova and Shimon Weiss}, journal={Proceedings of SPIE--the International Society for Optical Engineering}, year={2010}, volume={7608 76082D} }