High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire

@article{DeKosky2013HighthroughputSO,
  title={High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire},
  author={Brandon J. DeKosky and Gregory C Ippolito and Ryan P Deschner and Jason J. Lavinder and Yariv Wine and Brandon M. Rawlings and Navin Varadarajan and Claudia Giesecke and Thomas Doerner and Sarah F Andrews and Patrick C. Wilson and Scott P. Hunicke-Smith and C Grant Willson and Andrew D. Ellington and George D. Georgiou},
  journal={Nature Biotechnology},
  year={2013},
  volume={31},
  pages={166-169}
}
Each B-cell receptor consists of a pair of heavy and light chains. High-throughput sequencing can identify large numbers of heavy- and light-chain variable regions (VH and VL) in a given B-cell repertoire, but information about endogenous pairing of heavy and light chains is lost after bulk lysis of B-cell populations. Here we describe a way to retain this pairing information. In our approach, single B cells (>5 × 104 capacity per experiment) are deposited in a high-density microwell plate (125… CONTINUE READING
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