High-throughput sequencing of kDNA amplicons for the analysis of Leishmania minicircles and identification of Neotropical species.

@article{Kocher2018HighthroughputSO,
  title={High-throughput sequencing of kDNA amplicons for the analysis of Leishmania minicircles and identification of Neotropical species.},
  author={Arthur Kocher and Sophie Vali{\`e}re and Anne-Laure Ba{\~n}uls and J{\'e}r{\^o}me Murienne},
  journal={Parasitology},
  year={2018},
  volume={145 5},
  pages={
          585-594
        }
}
SUMMARYLeishmania kinetoplast DNA contains thousands of small circular molecules referred to as kinetoplast DNA (kDNA) minicercles. kDNA minicircles are the preferred targets for sensitive Leishmania detection, because they are present in high copy number and contain conserved sequence blocks in which polymerase chain reaction (PCR) primers can be designed. On the other hand, the heterogenic nature of minicircle networks has hampered the use of this peculiar genomic region for strain typing… CONTINUE READING

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