High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency

@inproceedings{Takahashi2016HighTS,
  title={High throughput sequencing analysis of RNA libraries reveals the influences of initial library and PCR methods on SELEX efficiency},
  author={Mayumi Takahashi and Xiwei Wu and Michelle Ho and Pritsana Chomchan and John J. Rossi and John C. Burnett and J Y Zhou},
  booktitle={Scientific reports},
  year={2016}
}
The systemic evolution of ligands by exponential enrichment (SELEX) technique is a powerful and effective aptamer-selection procedure. However, modifications to the process can dramatically improve selection efficiency and aptamer performance. For example, droplet digital PCR (ddPCR) has been recently incorporated into SELEX selection protocols to putatively reduce the propagation of byproducts and avoid selection bias that result from differences in PCR efficiency of sequences within the… CONTINUE READING
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