High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications.

@article{Stockwell1999HighthroughputSO,
  title={High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications.},
  author={B. Stockwell and S. Haggarty and S. Schreiber},
  journal={Chemistry & biology},
  year={1999},
  volume={6 2},
  pages={
          71-83
        }
}
BACKGROUND Fully adapting a forward genetic approach to mammalian systems requires efficient methods to alter systematically gene products without prior knowledge of gene sequences, while allowing for the subsequent characterization of these alterations. Ideally, these methods would also allow function to be altered in a temporally controlled manner. RESULTS We report the development of a miniaturized cell-based assay format that enables a genetic-like approach to understanding cellular… Expand
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