High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles

@article{Moller2007HighthroughputSO,
  title={High-throughput screening of monoclonal antibodies against plant cell wall glycans by hierarchical clustering of their carbohydrate microarray binding profiles},
  author={Isabel Moller and Susan E. Marcus and Ash Haeger and Yves Verhertbruggen and Ren{\'e} Verhoef and H A Schols and Peter Ulvskov and J\orn Dalgaard Mikkelsen and J Paul Knox and William W G T Willats},
  journal={Glycoconjugate Journal},
  year={2007},
  volume={25},
  pages={37 - 48}
}
Antibody-producing hybridoma cell lines were created following immunisation with a crude extract of cell wall polymers from the plant Arabidopsis thaliana. In order to rapidly screen the specificities of individual monoclonal antibodies (mAbs), their binding to microarrays containing 50 cell wall glycans immobilized on nitrocellulose was assessed. Hierarchical clustering of microarray binding profiles from newly produced mAbs, together with the profiles for mAbs with previously defined… CONTINUE READING

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References

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Showing 1-10 of 45 references

Localization of cell wall proteins in relation to the developmental anatomy of the carrot root apex

M. Smallwood, A. Beven, +4 authors J. P. Knox
Plant J. 5, 237–246 (1994) 48 Glycoconj J • 2008
View 1 Excerpt

Pectin: New insights into an old polymer are starting to gel

W.G.T. Willats, J. P. Knox, J. D. Mikkelsen
Trends Food Sci. Technol. 17(3), 97–104 • 2006
View 1 Excerpt

Monoclonal antibodies to plant cell wall xylans and arabinoxylans.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society • 2005
View 3 Excerpts

Primary cell wall metabolism: Tracking the careers of wall polymers in living plant cells

S. C. Fry
New Phytol. 161, 641–675 • 2004
View 1 Excerpt

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