High-throughput screening of chromatographic separations: IV. Ion-exchange.


Ion-exchange (IEX) chromatography steps are widely applied in protein purification processes because of their high capacity, selectivity, robust operation, and well-understood principles. Optimization of IEX steps typically involves resin screening and selection of the pH and counterion concentrations of the load, wash, and elution steps. Time and material… (More)
DOI: 10.1002/bit.21905


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