High-throughput screening methodology for the directed evolution of glycosyltransferases

@article{Aharoni2006HighthroughputSM,
  title={High-throughput screening methodology for the directed evolution of glycosyltransferases},
  author={Amir Aharoni and Karena Thieme and Cecilia Pui Chi Chiu and Sabrina Buchini and Luke L Lairson and Hongming Chen and Natalie C. J. Strynadka and Warren Wakarchuk and Stephen G Withers},
  journal={Nature Methods},
  year={2006},
  volume={3},
  pages={609-614}
}
Engineering of glycosyltransferases (GTs) with desired substrate specificity for the synthesis of new oligosaccharides holds great potential for the development of the field of glycobiology. However, engineering of GTs by directed evolution methodologies is hampered by the lack of efficient screening systems for sugar-transfer activity. We report here the development of a new fluorescence-based high-throughput screening (HTS) methodology for the directed evolution of sialyltransferases (STs… CONTINUE READING

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Glycosyltransferase engineering for carbohydrate synthesis.

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