High-throughput screening goes to school

  title={High-throughput screening goes to school},
  author={Alan W. Dove},
  journal={Nature Methods},
  • A. Dove
  • Published 1 June 2007
  • Biology
  • Nature Methods
High-throughput screening, traditionally the domain of big pharmaceutical companies, is now creeping into academic laboratories in the guise of chemical genomics. Though the technique can be demanding and expensive, it is already yielding impressive results, as Alan Dove finds out. 

Towards a comprehensive open source platform for management and analysis of High Content Screening data

This article presents two open source components that can be used as flexible and powerful building blocks for a monolithic data pipelines for High Content Screening investigations.

The Role of Chemical Biology in Drug Discovery

This article will highlight some critical contributions from chemical biology methods in the context of a high-level overview of drug discovery as practiced currently.

Ligand screening using enzymatic assays.

The various stages of screening are described, using a viral protease, NS3/4A from Hepatitis C virus, as an example of an enzyme target, and the ligand screening data is processed and analyzed using various strategies.

Hemozoin and antimalarial drug discovery.

The current approaches and limitations of high-throughput screening discovery of hemozoin inhibitors are discussed, and new methods must be developed to validate the mechanism of action of these hit compounds within the malaria parasite.

Genomics as knowledge enterprise: Implementing an electronic research habitat at the Biopolis Experimental Therapeutics Center

The Electronic Research Habitat at ETC is described, a comprehensive hardware and software infrastructure designed to effectively manage terabyte data flows and storage, increase back office efficiency, enhance the scientific work experience, and satisfy rigorous regulatory and legal requirements.

Computational approaches to developing short cyclic peptide modulators of protein-protein interactions.

This chapter will describe various computational strategies for virtual screening cyclic peptides, along with known implementations and applications, and explore the generation and screening of diverse combinatorial virtual libraries, incorporating a range of cyclization strategies and structural modifications.

The fall and rise of pharmacology--(re-)defining the discipline?

Workflow-Based Software Environment for Large-Scale Biological Experiments

An open-source research tool is introduced that allows for the management of the entire data flow of the HCS data chain, by handling and linking information and providing many powerful postprocessing and visualization tools.

An Oil-Free Picodrop Bioassay Platform for Synthetic Biology

Print Droplet Microfluidics is applied to construct defined reactions with chemicals and cells incubated under air on an open array to allow quantitation of hydrophobic compounds in compartmentalized reactors.



Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries.

The feasibility of qHTS for accurately profiling every compound in large chemical libraries (>10(5) compounds) is demonstrated, thereby providing a platform for chemical genomics and accelerating the identification of leads for drug discovery.

Proteome survey reveals modularity of the yeast cell machinery

This study reports the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry and provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.

Small molecules that reactivate p53 in renal cell carcinoma reveal a NF-kappaB-dependent mechanism of p53 suppression in tumors.

It is suggested that the complete or partial repression of p53 observed in many tumors can be the result of constitutive activation of NF-kappaB, and anticancer applications for the well known antimalaria drug quinacrine are suggested.

Proc. Natl. Acad. Sci. USA 103

  • Proc. Natl. Acad. Sci. USA 103
  • 2006

Proc. Natl. Acad. Sci. USA 102

  • Proc. Natl. Acad. Sci. USA 102
  • 2005

Alan Dove is a science writer based in the New York City area (alan.dove@gmail. com)

  • Alan Dove is a science writer based in the New York City area (alan.dove@gmail. com)