High-throughput screening for monoamine oxidase-A and monoamine oxidase-B inhibitors using one-step fluorescence assay

@article{Guang2006HighthroughputSF,
  title={High-throughput screening for monoamine oxidase-A and monoamine oxidase-B inhibitors using one-step fluorescence assay},
  author={Hong-mei Guang and Guan-hua Du},
  journal={Acta Pharmacologica Sinica},
  year={2006},
  volume={27},
  pages={760-766}
}
AbstractAim:To develop high-throughput screening (HTS) assays for monoamine oxidase (MAO)-A and MAO-B inhibitors.Methods:A fluorescence probe based method measuring MAO-A and MAO-B activity was established and optimized, with its sensitivity, stability and specificity evaluated. Reaction conditions including enzyme sources, substrate concentrations, incubation volume and reaction time in 384-well format were optimized to achieve sensitive and low consumptive goal.Results:In optimized conditions… CONTINUE READING