High-throughput screening for inhibitors of the e3 ubiquitin ligase APC.

  title={High-throughput screening for inhibitors of the e3 ubiquitin ligase APC.},
  author={Jianing Huang and Julie Sheung and Guoqiang Dong and Christina Coquilla and Sarkiz Daniel-Issakani and Donald G. Payan},
  journal={Methods in enzymology},
The anaphase-promoting complex (APC) is an E3 ubiquitin ligase that mediates the ubiquitination and degradation of the securin protein and mitotic cyclins, resulting in the regulation of the onset of sister-chromatid separation and mitotic exit. In an effort to identify novel therapeutic compounds that modulate cell proliferation and, therefore, have potential applications in oncology, a plate-based in vitro ubiquitination assay that uses recombinant purified E1, E2 (UbcH5c), E3 (APC11/APC2… CONTINUE READING


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