High-throughput screening assay for embryoid body differentiation of human embryonic stem cells.

@article{Outten2012HighthroughputSA,
  title={High-throughput screening assay for embryoid body differentiation of human embryonic stem cells.},
  author={Joel T Outten and Paul Gadue and Deborah L. French and Scott L. Diamond},
  journal={Current protocols in stem cell biology},
  year={2012},
  volume={Chapter 1},
  pages={Unit 1D.6}
}
Serum-free human pluripotent stem cell media offer the potential to develop reproducible clinically applicable differentiation strategies and protocols. The vast array of possible growth factor and cytokine combinations for media formulations makes differentiation protocol optimization both labor and cost-intensive. This unit describes a 96-well plate, 4-color flow cytometry-based screening assay to optimize pluripotent stem cell differentiation protocols. We provide conditions both to… CONTINUE READING