High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization.

@article{Yoshikawa2006HighthroughputSF,
  title={High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization.},
  author={Toshiyuki Yoshikawa and Y. Piao and J. Zhong and R. Matoba and M. G. Carter and Yuxia Wang and Ilya Goldberg and M. Ko},
  journal={Gene expression patterns : GEP},
  year={2006},
  volume={6 2},
  pages={
          213-24
        }
}
Mammalian preimplantation embryos provide an excellent opportunity to study temporal and spatial gene expression in whole mount in situ hybridization (WISH). However, large-scale studies are made difficult by the size of the embryos ( approximately 60mum diameter) and their fragility. We have developed a chamber system that allows parallel processing of embryos without the aid of a microscope. We first selected 91 candidate genes that were transcription factors highly expressed in blastocysts… Expand
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References

SHOWING 1-10 OF 49 REFERENCES
A global view of gene expression in the preimplantation mouse embryo: morula versus blastocyst.
  • Tetsuya S. Tanaka, M. Ko
  • Biology, Medicine
  • European journal of obstetrics, gynecology, and reproductive biology
  • 2004
TLDR
Global gene-expression profiling of morula and blastocyst using the NIA 15k mouse cDNA microarray identified 428 genes upregulated and 748 downregulated in Blastocyst compared to morula, consistent with semi-quantative RT-PCR analysis of sample genes. Expand
GEISHA, a whole‐mount in situ hybridization gene expression screen in chicken embryos
TLDR
Results show that high throughput whole‐mount in situ hybridization (ISH) provides an effective approach for identifying novel genes that are differentially expressed in the developing chicken embryo. Expand
Large-scale expression screening by automated whole-mount in situ hybridization
TLDR
A time and cost efficient high-throughput whole-mount in situ technique to perform a large-scale gene expression analysis in medaka fish (Oryzias latipes) embryos is presented. Expand
A gene expression screen in zebrafish embryogenesis.
TLDR
The usefulness of the in situ hybridization screening approach is illustrated by describing several new markers for the characteristic structure in the fish embryo named the yolk syncytial layer, and for different regions of the developing brain. Expand
Transcriptome Analysis of Mouse Stem Cells and Early Embryos
TLDR
The sequences and cDNA clones recovered in this work provide a comprehensive resource for genes functioning in early mouse embryos and stem cells, and nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine. Expand
Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development.
Little is known about gene action in the preimplantation events that initiate mammalian development. Based on cDNA collections made from each stage from egg to blastocyst, 25438 3'-ESTs were derived,Expand
Transcript profiling during preimplantation mouse development.
TLDR
Using microarrays, EASE identifies genes involved in critical biological processes that can be the subject of a more traditional hypothesis-driven approach and validates the hypothesis-generating approach. Expand
Requirement for Foxd3 in maintaining pluripotent cells of the early mouse embryo.
TLDR
It is reported that Foxd3, a member of the forkhead family of transcriptional regulators, is required for maintenance of embryonic cells of the early mouse embryo and thatFoxd3-/- embryos can be rescued by a small number of wild-type cells. Expand
In situ-synthesized novel microarray optimized for mouse stem cell and early developmental expression profiling.
TLDR
This work has developed a microarray platform containing in situ-synthesized 60-mer oligonucleotide probes representing approximately 22,000 unique mouse transcripts, assembled primarily from sequences of stem cell and embryo cDNA libraries. Expand
The early embryonic expression of TFII-I during mouse preimplantation development.
TLDR
The early embryonic expression of mouse TFII-I implicates this family of transcription factors in preimplantation development, and using RT-PCR detected Gtf2i and GTF2ird1 mRNA transcripts in unfertilized oocytes, which indicates the maternal expression of these genes. Expand
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5
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