High-throughput scintillation proximity assay for transglutaminase activity measurement.

  title={High-throughput scintillation proximity assay for transglutaminase activity measurement.},
  author={Andr{\'a}s M{\'a}di and Levente K{\'a}rp{\'a}ti and Andr{\'a}s Kov{\'a}cs and L{\'a}szl{\'o} Muszbek and L{\'a}szl{\'o} F{\'e}s{\"u}s},
  journal={Analytical biochemistry},
  volume={343 2},
Members of the transglutaminase enzyme family are involved in a broad range of biological phenomena, including haemostasis, apoptosis, semen coagulation, skin formation, and wound healing. A new and rapid method for measurement of transglutaminase activity is described in this article. The enzyme links tritium-labeled putrescine to biotinylated oligoglutamine, and the tritiated peptide is bound to a streptavidin-coated microtiter plate permanently covered by a thin layer of scintillant. Only… CONTINUE READING