High-throughput quantification of early stages of phagocytosis.

@article{Yeo2013HighthroughputQO,
  title={High-throughput quantification of early stages of phagocytosis.},
  author={Jeremy Changyu Yeo and Adam A Wall and Jennifer L Stow and Nicholas A. Hamilton},
  journal={BioTechniques},
  year={2013},
  volume={55 3},
  pages={
          115-24
        }
}
Phagocytosis--the engulfment of cells and foreign bodies--is an important cellular process in innate immunity, development, and disease. Quantification of various stages of phagocytosis, especially in a rapid screening fashion, is an invaluable tool for elucidating protein function during this process. However, current methods for assessing phagocytosis are largely limited to flow cytometry and manual image-based assays, providing limited information. Here, we present an image-based, semi… CONTINUE READING

Figures and Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-9 OF 9 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 11 REFERENCES

A novel method to determine the engulfment of apoptotic cells by macrophages using pHrodo succinimidyl ester

M. Miksa, H. Kornura, R. Wu, K. G. Shah, P. Wang
  • J. Immunol. Methods
  • 2009

Quantitative analysis of membrane remodeling at phagocytic cup

D. A. G. S. Hume
  • Mol . Biol . Cell
  • 2007