High-throughput protein precipitation and hydrophobic interaction chromatography: salt effects and thermodynamic interrelation.

Abstract

Salt-induced protein precipitation and hydrophobic interaction chromatography (HIC) are two widely used methods for protein purification. In this study, salt effects in protein precipitation and HIC were investigated for a broad combination of proteins, salts and HIC resins. Interrelation between the critical thermodynamic salting out parameters in both… (More)
DOI: 10.1016/j.chroma.2011.08.016

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