High-throughput mapping of a whole rhesus monkey brain at micrometer resolution.

  title={High-throughput mapping of a whole rhesus monkey brain at micrometer resolution.},
  author={Fang Xu and Yan Shen and Lufeng Ding and Chaoyu Yang and Heng Tan and Hao Wang and Qingyuan Zhu and Rui Xu and Feng Wu and Yanyang Xiao and Cheng Xu and Qianwei Li and Peng Su and Li I. Zhang and Hong-wei Dong and Robert Desimone and Fuqiang Xu and Xintian Hu and Pak-Ming Lau and Guo-Qiang Bi},
  journal={Nature biotechnology},
Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and the relatively low throughput of available microscopy methods. Here, we present an approach that combines primate-optimized tissue sectioning and clearing with ultrahigh-speed fluorescence microscopy implementing improved volumetric imaging with synchronized on-the-fly-scan and readout technique, and is capable of completing whole-brain imaging of a rhesus monkey at 1 × 1 × 2.5 µm3 voxel resolution within 100 h… 

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