High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) involved in non-host resistance and R gene-mediated resistance.

@article{Coemans2008HighthroughputIP,
  title={High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) involved in non-host resistance and R gene-mediated resistance.},
  author={Bert Coemans and Yoshihiro Takahashi and Thomas Berberich and Akiko Ito and Hiroyuki Kanzaki and Hideo Matsumura and Hiromasa Saitoh and Shinya Tsuda and Sophien Kamoun and L{\'a}szl{\'o} S{\'a}gi and Rony Swennen and Ryohei Terauchi},
  journal={Molecular plant pathology},
  year={2008},
  volume={9 1},
  pages={25-36}
}
To identify positive regulators of cell death in plants, we performed a high-throughput screening, employing potato virus X-based overexpression in planta of a cDNA library derived from paraquat-treated Nicotiana benthamiana leaves. The screening of 30,000 cDNA clones enabled the identification of an ADP-ribosylation factor 1 (ARF1) that induces cell death when overexpressed in N. benthamiana. Overexpression of the guanosine diphosphate (GDP)-locked mutant of ARF1 did not trigger cell death… CONTINUE READING

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