High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking.

Abstract

We have developed a method combining microfluidics, time-lapsed single-molecule microscopy and automated image analysis allowing for the observation of an excess of 3000 complete cell cycles of exponentially growing Escherichia coli cells per experiment. The method makes it possible to analyse the rate of gene expression at the level of single proteins over… (More)
DOI: 10.1098/rstb.2012.0025

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