High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking.

@article{Ullman2013HighthroughputGE,
  title={High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking.},
  author={G Ullman and Mats Walld{\'e}n and Erik G Marklund and Anel Mahmutovic and Ivan A Razinkov and Johan Elf},
  journal={Philosophical transactions of the Royal Society of London. Series B, Biological sciences},
  year={2013},
  volume={368 1611},
  pages={20120025}
}
We have developed a method combining microfluidics, time-lapsed single-molecule microscopy and automated image analysis allowing for the observation of an excess of 3000 complete cell cycles of exponentially growing Escherichia coli cells per experiment. The method makes it possible to analyse the rate of gene expression at the level of single proteins over the bacterial cell cycle. We also demonstrate that it is possible to count the number of non-specifically DNA binding LacI-Venus molecules… CONTINUE READING
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Robust Growth of Escherichia coli

Current Biology • 2010
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