High-throughput fluorescence polarization assay for chemical library screening against anti-apoptotic Bcl-2 family member Bfl-1.

Abstract

Overexpression of the anti-apoptotic Bcl-2 family proteins occurs commonly in human cancers. Bfl-1 is highly expressed in some types of malignant cells, contributing significantly to tumor cell survival and chemoresistance. Therefore, it would be desirable to have chemical antagonists of Bfl-1. To this end, we devised a fluorescence polarization assay (FPA… (More)
DOI: 10.1177/1087057111429372

Topics

Cite this paper

@article{Zhai2012HighthroughputFP, title={High-throughput fluorescence polarization assay for chemical library screening against anti-apoptotic Bcl-2 family member Bfl-1.}, author={Dayong Zhai and Paulo H C Godoi and Eduard A. Sergienko and Russell S Dahl and Xochella Chan and Brock T Brown and Justin Rascon and Andrew Hurder and Ying Su and Thomas D Y Chung and Chaofang Jin and Paul W. Diaz and John C. Reed}, journal={Journal of biomolecular screening}, year={2012}, volume={17 3}, pages={350-60} }