High-throughput development of a hybrid-type fluorescent glutamate sensor for analysis of synaptic transmission.


Fluorescent sensors are powerful tools for visualizing cellular molecular dynamics. We present a high-throughput screening system, designated hybrid-type fluorescence indicator development (HyFInD), to identify optimal position-specific fluorophore labeling in hybrid-type sensors consisting of combinations of ligand-binding protein mutants with small… (More)
DOI: 10.1002/anie.201407181


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