High-throughput capillary electrophoresis for the identification and differentiation of seven species of Eimeria from chickens.

@article{Gasser2005HighthroughputCE,
  title={High-throughput capillary electrophoresis for the identification and differentiation of seven species of Eimeria from chickens.},
  author={Robin B. Gasser and Rob Skinner and Reza Fadavi and Grant Richards and Genevieve Morris},
  journal={Electrophoresis},
  year={2005},
  volume={26 18},
  pages={3479-85}
}
A capillary electrophoretic approach has been evaluated for the identification of seven currently recognised species of Eimeria infecting chickens. The second internal transcribed spacer of ribosomal DNA is PCR-amplified from any of the seven species using a single set of oligonucleotide primers (one of which is fluorescently labelled). The amplicons are heat-denatured and subjected to capillary electrophoresis in a MegaBACE 1000 (Amersham). The chromatograms captured are stored electronically… CONTINUE READING

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