High-throughput array production using precision glass syringes.

@article{Shieh2002HighthroughputAP,
  title={High-throughput array production using precision glass syringes.},
  author={Jean Shieh and C To and J Carramao and Nobuya Nishimura and Yukio Maruta and Yuki Hashimoto and David Wright and H. C. Wu and Arezou Azarani},
  journal={BioTechniques},
  year={2002},
  volume={32 6},
  pages={1360-2, 1364-5}
}
The advantages of using 1, 96, or 384 precision glass syringes in automated high-throughput microdispensers in creating highly uniform and reproducible DNA, protein, and organic compound array filters and slides are described. Using the Hydra Microdispenser and Tango Liquid Handling system, 0.1-5 ng (in 50-300 nL) PCR-amplified, human cancer-related genes and housekeeping genes were spotted onto nylon membranes and coated slides. Protein solutions of 50 microg/mL to 1 mg/mL were spotted onto… CONTINUE READING

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