High-throughput absolute quantification of proteins using an improved two-dimensional reversed-phase separation and quantification concatemer (QconCAT) approach.

@article{Wei2014HighthroughputAQ,
  title={High-throughput absolute quantification of proteins using an improved two-dimensional reversed-phase separation and quantification concatemer (QconCAT) approach.},
  author={Junying Wei and Chen Ding and Jiao Zhang and Wei Mi and Yan Zhao and Mingwei Liu and Tianyi Fu and Yangjun Zhang and Wantao Ying and Yun Cai and Jun Qin and Xiaohong Qian},
  journal={Analytical and bioanalytical chemistry},
  year={2014},
  volume={406 17},
  pages={4183-93}
}
Stable isotope dilution-selective reaction monitoring-mass spectrometry (SID-SRM-MS) has been widely used for the absolute quantitative analysis of proteins. However, when performing the large-scale absolute quantification of proteins from a more complex tissue sample, such as mouse liver, in addition to a high-throughput approach for the preparation and calibration of large amounts of stable-isotope-labelled internal standards, a more powerful separation method prior to SRM analysis is also… CONTINUE READING