High-throughput SNP genotyping by single-tube PCR with Tm-shift primers.

@article{Wang2005HighthroughputSG,
  title={High-throughput SNP genotyping by single-tube PCR with Tm-shift primers.},
  author={Jun Wang and Karen Chuang and Mandeep Ahluwalia and Sarika S Patel and Nanette Umblas and Daniel B. Mirel and Russell Higuchi and Soren Germer},
  journal={BioTechniques},
  year={2005},
  volume={39 6},
  pages={
          885-93
        }
}
Despite many recent advances in high-throughput single nucleotide polymorphism (SNP) genotyping technologies, there is still a great need for inexpensive and flexible methods with a reasonable throughput. Here we report substantial modifications and improvements to an existing homogenous allele-specific PCR-based SNP genotyping method, making it an attractive new option for researchers engaging in candidate gene studies or following up on genome-wide scans. In this advanced version of the… CONTINUE READING