High-throughput RNAi screening in vitro: from cell lines to primary cells.

  title={High-throughput RNAi screening in vitro: from cell lines to primary cells.},
  author={Dmitriy Ovcharenko and R. Jarvis and Scott P. Hunicke-Smith and Kevin Kelnar and David Benjamin Brown},
  volume={11 6},
Small interfering RNAs (siRNAs) are being used to induce sequence-specific gene silencing in cultured cells to study mammalian gene function. Libraries of siRNAs targeting entire human gene classes can be used to identify genes with specific cellular functions. Here we describe high-throughput siRNA delivery methods to facilitate siRNA library screening experiments with both immortalized and primary cells. We adapted chemical reverse transfection for immortalized adherent cell lines in a 96… CONTINUE READING
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Delivering siRNAs to difficult cell types

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