High-throughput RNA interference screens in Drosophila tissue culture cells.

@article{Armknecht2005HighthroughputRI,
  title={High-throughput RNA interference screens in Drosophila tissue culture cells.},
  author={Susan Armknecht and Michael Boutros and Amy A Kiger and Kent Nybakken and Bernard Mathey-Prevot and Norbert Perrimon},
  journal={Methods in enzymology},
  year={2005},
  volume={392},
  pages={55-73}
}
This chapter describes the method used to conduct high-throughput screening (HTs) by RNA interference in Drosophila tissue culture cells. It covers four main topics: (1) a brief description of the existing platforms to conduct RNAi-screens in cell-based assays; (2) a table of the Drosophila cell lines available for these screens and a brief mention of the need to establish other cell lines as well as cultures of primary cells; (3) a discussion of the considerations and protocols involved in… CONTINUE READING