High-throughput DNA analysis by time-of-flight mass spectrometry

  title={High-throughput DNA analysis by time-of-flight mass spectrometry},
  author={Joseph Albert Monforte and Christopher Becker},
  journal={Nature Medicine},

Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems.

This commonly used genotyping technique is introduced and described, providing an overview on available resources on STRs, multiplex design, and analysis.

Measuring specificity in multi-substrate/product systems as a simple tool to investigate selectivity in vivo

This review looks at different methods for studying multiple substrate enzymes, as well as explores options on how to most accurately describe an enzyme’s activity within these multi-substrate systems.

Detection of Genetic Alterations by Nucleic Acid Analysis: Use of PCR and Mass Spectroscopy-Based Methods

This review focuses on the PCR and Mass-spectrometry methods developed to detect circulating DNA alteration from blood, in evolving applications such as cancer diagnostic tools and gives advice and guidelines for designing PCR experiments with the specific requirements of circulating DNA.

Next Generation DNA Sequencing (II): Techniques, Applications

The status of the technology in the second half of the year 2015, both of the novel commercially available platforms, and of the systems in development are discussed, and techniques with potential for future DNA sequencing techniques are mentioned.

New Horizons in Next-Generation Sequencing

This chapter describes the most common and well-established next-generation sequencing technologies and platforms and several methodologies and sequencers with outstanding features that have also been released in the last few years.

Application of mass spectrometry to molecular diagnostics of viral infections

Matching of molecular techniques with specific requirements of the major MS platforms has produced powerful technologies for molecular diagnostics, which will further benefit from coupling with computational tools for extracting clinical information from MS-derived data.



Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.

  • J. Clark
  • Biology, Chemistry
    Nucleic acids research
  • 1988
Results demonstrate that template instruction is not an absolute requirement for the catalysis of nucleotidyl transfer reactions by DNA polymerases.

Matrix-assisted laser desorption mass spectrometry of oligodeoxythmidylic acids

Ferulic acid, sinapinic acid and 2,5-dihydroxybenzoic acid (DHBA) have been tested as matrix materials for matrix-assisted laser desorption of the pure oligonucleotide pd(T)12 and a mixture of

Interpretation of Mass Spectra

1. Introduction 2. Elemental Composition 3. The Molecular Ion 4. Basic Mechanisms of Ion Fragmentation 5. Postulation of Molecular Structures 6. Auxiliary Techniques 7. Theory of Unimolecular Ion

Time-of-flight mass spectrometry of underivatized single-stranded DNA oligomers by matrix-assisted laser desorption.

It is concluded that a linear TOF system is better suited for the mass spectrometric analysis of ssDNA oligomers larger than about a 25-mer, with the apparent advantages of better sensitivity and mass resolution.

Effect of impurities on the matrix-assisted laser desorption mass spectra of single-stranded oligodeoxynucleotides.

The effect of impurities on the analysis of single-stranded DNA oligomers by the technique of matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry has been studied using

Molecular Detection of Primary Bladder Cancer by Microsatellite Analysis

In a blinded study, urine samples from 25 patients with suspicious bladder lesions that had been identified cystoscopically were analyzed and microsatellite changes matching those in the tumor were detected in the urine sediment of 19 of the 20 patients who were diagnosed with bladder cancer.

Genotyping of forensic short tandem repeat (STR) systems based on sizing precision in a capillary electrophoresis instrument

The precision of the 310 capillary electrophoresis system, coupled with software developed for automated genotyping of alleles based on the use of an allelie ladder, allows for accurate genotypesing of STR loci.

Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons.

In the following, the first results on ultraviolet laser desorption (UVLD) of bioorganic compounds in the mass range above 10000 daltons are reported. Strong molecular ion signals were registered by