High-throughput DNA analysis by time-of-flight mass spectrometry

@article{Monforte1997HighthroughputDA,
  title={High-throughput DNA analysis by time-of-flight mass spectrometry},
  author={J. Monforte and C. Becker},
  journal={Nature Medicine},
  year={1997},
  volume={3},
  pages={360-362}
}
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References

SHOWING 1-10 OF 14 REFERENCES
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
  • J. Clark
  • Biology, Medicine
  • Nucleic acids research
  • 1988
TLDR
Results demonstrate that template instruction is not an absolute requirement for the catalysis of nucleotidyl transfer reactions by DNA polymerases. Expand
Genotyping of forensic short tandem repeat (STR) systems based on sizing precision in a capillary electrophoresis instrument
TLDR
The precision of the 310 capillary electrophoresis system, coupled with software developed for automated genotyping of alleles based on the use of an allelie ladder, allows for accurate genotypesing of STR loci. Expand
Effect of impurities on the matrix-assisted laser desorption mass spectra of single-stranded oligodeoxynucleotides.
The effect of impurities on the analysis of single-stranded DNA oligomers by the technique of matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry has been studied usingExpand
Molecular Detection of Primary Bladder Cancer by Microsatellite Analysis
TLDR
In a blinded study, urine samples from 25 patients with suspicious bladder lesions that had been identified cystoscopically were analyzed and microsatellite changes matching those in the tumor were detected in the urine sediment of 19 of the 20 patients who were diagnosed with bladder cancer. Expand
Time-of-flight mass spectrometry of underivatized single-stranded DNA oligomers by matrix-assisted laser desorption.
TLDR
It is concluded that a linear TOF system is better suited for the mass spectrometric analysis of ssDNA oligomers larger than about a 25-mer, with the apparent advantages of better sensitivity and mass resolution. Expand
Automated DNA profiling employing multiplex amplification of short tandem repeat loci.
TLDR
The combination of multiplex STR-PCR and automatic fluorescence-based detection is thus a rapid and powerful technique for individual identification. Expand
Matrix-assisted laser desorption time-of-flight mass spectrometry of oligonucleotides using 3-hydroxypicolinic acid as an ultraviolet-sensitive matrix.
TLDR
Results were obtained with a new matrix material, 3-hydroxypicolinic acid (3-Hydroxypyridine-2-carboxylic acid), which showed significant improvement over many previously reported matrices studied in terms of mass range available, signal-to-noise ratio, and the ability to analyze mixed-base oligomers. Expand
Matrix-assisted laser desorption mass spectrometry of oligodeoxythmidylic acids
Ferulic acid, sinapinic acid and 2,5-dihydroxybenzoic acid (DHBA) have been tested as matrix materials for matrix-assisted laser desorption of the pure oligonucleotide pd(T)12 and a mixture ofExpand
Principles and applications of matrix-assisted UV-laser desorption/ionization mass spectrometry
Abstract Matrix-assisted laser desorption/ionization mass spectrometry (LDI MS) has shown its potential for desorbing ions of biomolecules with relative molecular masses ( M r ) up to about 300 000Expand
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