High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay

@inproceedings{Yucha2017HighthroughputCO,
  title={High-throughput Characterization of HIV-1 Reservoir Reactivation Using a Single-Cell-in-Droplet PCR Assay},
  author={Robert W. Yucha and Kristen S Hobbs and Emily Hanhauser and Louise E Hogan and Wildaliz Nieves and Mehmet Ozgun Ozen and Fatih Inci and Vanessa A. York and Erica A Gibson and Cassandra Thanh and Hadi Shafiee and Rami El Assal and Maja Kiselinova and Yvonne P. Robles and Helen Bae and Kaitlyn S. Leadabrand and Shuqi Wang and Steven G. Deeks and Daniel R. Kuritzkes and Utkan Demirci and Timothy J Henrich},
  booktitle={EBioMedicine},
  year={2017}
}
Reactivation of latent viral reservoirs is on the forefront of HIV-1 eradication research. However, it is unknown if latency reversing agents (LRAs) increase the level of viral transcription from cells producing HIV RNA or harboring transcriptionally-inactive (latent) infection. We therefore developed a microfluidic single-cell-in-droplet (scd)PCR assay to directly measure the number of CD4+ T cells that produce unspliced (us)RNA and multiply spliced (ms)RNA following ex vivo latency reversal… CONTINUE READING

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