High-throughput Assays for Superoxide and Hydrogen Peroxide

  title={High-throughput Assays for Superoxide and Hydrogen Peroxide},
  author={Jacek Zielonka and Gang Cheng and Monika Zielonka and Thota Ganesh and Aiming Sun and Joy Joseph and Radosław Michalski and William James O'brien and John David Lambeth and Balaraman Kalyanaraman},
  journal={The Journal of Biological Chemistry},
  pages={16176 - 16189}
Background: NADPH oxidases (Nox) are involved in pathogenesis of inflammatory and fibrotic diseases. Results: High-throughput screening methodology is developed for discovery and determination of Nox inhibitors and mechanism. Conclusion: Rapid and specific monitoring of superoxide and hydrogen peroxide is possible, enabling high-throughput screening of Nox isoform-specific inhibitors. Significance: The proposed strategy will enable more rigorous research on the chemical biology of Nox enzymes… 

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