High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array.

  title={High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array.},
  author={Yi-Chung Tung and Amy Y. Hsiao and Steven G Allen and Yu-suke Torisawa and Mitchell Ho and Shuichi Takayama},
  journal={The Analyst},
  volume={136 3},
Culture of cells as three-dimensional (3D) aggregates can enhance in vitro tests for basic biological research as well as for therapeutics development. Such 3D culture models, however, are often more complicated, cumbersome, and expensive than two-dimensional (2D) cultures. This paper describes a 384-well format hanging drop culture plate that makes spheroid formation, culture, and subsequent drug testing on the obtained 3D cellular constructs as straightforward to perform and adapt to existing… CONTINUE READING
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