High-throughput, signature-tagged mutagenic approach to identify novel virulence factors of Yersinia pestis CO92 in a mouse model of infection.

@article{Ponnusamy2015HighthroughputSM,
  title={High-throughput, signature-tagged mutagenic approach to identify novel virulence factors of Yersinia pestis CO92 in a mouse model of infection.},
  author={Duraisamy Ponnusamy and Eric C. Fitts and Jian Sha and Tatiana E. Erova and Elena V. Kozlova and Michelle L. Kirtley and Bethany L. Tiner and Jourdan A. Andersson and Ashok Kumar Chopra},
  journal={Infection and immunity},
  year={2015},
  volume={83 5},
  pages={2065-81}
}
The identification of new virulence factors in Yersinia pestis and understanding their molecular mechanisms during an infection process are necessary in designing a better vaccine or to formulate an appropriate therapeutic intervention. By using a high-throughput, signature-tagged mutagenic approach, we created 5,088 mutants of Y. pestis strain CO92 and screened them in a mouse model of pneumonic plague at a dose equivalent to 5 50% lethal doses (LD50) of wild-type (WT) CO92. From this screen… CONTINUE READING