High-throughput, multiplexed IgG subclassing of antigen-specific antibodies from clinical samples.

@article{Brown2012HighthroughputMI,
  title={High-throughput, multiplexed IgG subclassing of antigen-specific antibodies from clinical samples.},
  author={Eric P. Brown and Anna F. Licht and Anne-Sophie Dugast and Ickwon Choi and Chris Bailey-Kellogg and Galit Alter and Margaret E. Ackerman},
  journal={Journal of immunological methods},
  year={2012},
  volume={386 1-2},
  pages={
          117-23
        }
}
In vivo, the activity of antibodies relies critically on properties of both the variable domain, responsible for antigen recognition, and the constant domain, responsible for innate immune recognition. Here, we describe a flexible, microsphere-based array format for capturing information about both functional ends of disease-specific antibodies from complex, polyclonal clinical serum samples. Using minimal serum, we demonstrate IgG subclass profiling of multiple antibody specificities. We… CONTINUE READING

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