High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases.

@article{Demian2009HighthroughputCL,
  title={High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases.},
  author={Douglas J Demian and Susan Clugston and Meta M Foster and Lucia E. Rameh and Deborah A. Sarkes and Sharon A Townson and Lily C Yang and Melvin Zhang and Maura E. Charlton},
  journal={Journal of biomolecular screening},
  year={2009},
  volume={14 7},
  pages={838-44}
}
Lipid kinases are central players in lipid signaling pathways involved in inflammation, tumorigenesis, and metabolic syndrome. A number of these kinase targets have proven difficult to investigate in higher throughput cell-free assay systems. This challenge is partially due to specific substrate interaction requirements for several of the lipid kinase family members and the resulting incompatibility of these substrates with most established, homogeneous assay formats. Traditional, cell-free in… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 23 references

divecha n: an emerging role for ptdins(4,5) p2-mediated signalling in human disease

JR Halstead, K Jalink
Trends Pharmacol Sci • 2005
View 7 Excerpts
Highly Influenced

Lipid signalling in disease

Nature Reviews Molecular Cell Biology • 2008

cloutier a, beaudet l, Roby p, et al: expression and purification of pi3 kinase α and development of an aTp depletion and an alphascreen pi3 kinase activity assay

boldyreff b, Rasmussen Tl, HH Jensen
J Biomol Screen 2008;13:1036-1040 • 2008

a, mcmillan K, et al: identification of small molecule ceramide kinase inhibitors using a homogeneous chemiluminescence high throughput assay

munagala n, nguyen s, W lam, J lee, Joly
Assay Drug Dev Technol • 2007
View 1 Excerpt

cantley lc : aKT / pKb signaling : navigating downstream

manning bd
Cell • 2007

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