High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases.

  title={High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases.},
  author={Douglas J Demian and Susan Clugston and Meta M Foster and Lucia E. Rameh and Deborah A. Sarkes and Sharon A Townson and Lily C Yang and Melvin Zhang and Maura E. Charlton},
  journal={Journal of biomolecular screening},
  volume={14 7},
Lipid kinases are central players in lipid signaling pathways involved in inflammation, tumorigenesis, and metabolic syndrome. A number of these kinase targets have proven difficult to investigate in higher throughput cell-free assay systems. This challenge is partially due to specific substrate interaction requirements for several of the lipid kinase family members and the resulting incompatibility of these substrates with most established, homogeneous assay formats. Traditional, cell-free in… CONTINUE READING


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