High spontaneous mutation frequency of BPV shuttle vector.

  title={High spontaneous mutation frequency of BPV shuttle vector.},
  author={Claire Ashman and R. L. Davidson},
  journal={Somatic cell and molecular genetics},
  volume={11 5},
A recombinant shuttle vector containing the entire bovine papillomavirus (BPV) genome, sequences from pBR322, and the Escherichia coli gpt gene was used to transform mouse C127 cells. Plasmid extracted from the transformed mouse cells was used to transform a Gpt- derivative of E. coli HB101, and the relative frequency of plasmids carrying a mutation in the gpt gene was determined. Approximate mutant frequencies of 3-16 X 10(-3) were observed for plasmid molecules which had been passaged through… CONTINUE READING

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