High-speed laser microsurgery of alert fruit flies for fluorescence imaging of neural activity.

@article{Sinha2013HighspeedLM,
  title={High-speed laser microsurgery of alert fruit flies for fluorescence imaging of neural activity.},
  author={Supriyo Sinha and Liang Liang and Eric Tatt Wei Ho and Karel E. Urbanek and Liqun Luo and Thomas M. Baer and Mark J. Schnitzer},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2013},
  volume={110 46},
  pages={18374-9}
}
Intravital microscopy is a key means of monitoring cellular function in live organisms, but surgical preparation of a live animal for microscopy often is time-consuming, requires considerable skill, and limits experimental throughput. Here we introduce a spatially precise (<1-µm edge precision), high-speed (<1 s), largely automated, and economical protocol… CONTINUE READING