High-speed force mapping on living cells with a small cantilever atomic force microscope.

@article{Braunsmann2014HighspeedFM,
  title={High-speed force mapping on living cells with a small cantilever atomic force microscope.},
  author={Christoph Braunsmann and Jan Seifert and Johannes Rheinlaender and Tilman E. Sch{\"a}ffer},
  journal={The Review of scientific instruments},
  year={2014},
  volume={85 7},
  pages={
          073703
        }
}
The imaging speed of the wide-spread force mapping mode for quantitative mechanical measurements on soft samples in liquid with the atomic force microscope (AFM) is limited by the bandwidth of the z-scanner and viscous drag forces on the cantilever. Here, we applied high-speed, large scan-range atomic force microscopy and small cantilevers to increase the speed of force mapping by ≈10-100 times. This allowed resolving dynamic processes on living mouse embryonic fibroblasts. Cytoskeleton… 
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References

SHOWING 1-10 OF 55 REFERENCES
Relative microelastic mapping of living cells by atomic force microscopy.
High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events
TLDR
This work describes the implementation of high-speed AFM coupled with an optical fluorescence microscope by developing a tip-scanning system, instead of a sample- scanning system, which operates on an inverted optical microscope, and conducted structural studies of living HeLa and 3T3 fibroblast cell surfaces.
A high-speed atomic force microscope for studying biological macromolecules
The atomic force microscope (AFM) is a powerful tool for imaging individual biological molecules attached to a substrate and placed in aqueous solution. At present, however, it is limited by the…
Atomic force microscopy probing of cell elasticity.
Wide-area scanner for high-speed atomic force microscopy.
TLDR
A wide-area scanner with a maximum XY scan range of ~46 × 46 μm is developed by magnifying the displacements of stack piezoelectric actuators using a leverage mechanism and the nonlinearity of the X- and Y-piezoelectic actuators' displacements that arises from their hysteresis is eliminated by polynomial-approximation-based open-loop control.
The simultaneous measurement of elastic, electrostatic and adhesive properties by scanning force microscopy: pulsed-force mode operation
We describe the pulsed-force mode, a new measuring mode for the scanning force microscope to image elastic, electrostatic and adhesive properties simultaneously with topography. The pulsed-force mode…
Dynamic effects on force measurements. I. Viscous drag on the atomic force microscope cantilever
When the atomic force microscope (AFM) is used for force measurements, the driving speed typically does not exceed a few microns per second. However, it is possible to perform the AFM force…
Cell Visco-Elasticity Measured with AFM and Optical Trapping at Sub-Micrometer Deformations
TLDR
Analysis of the response of single 3T3 fibroblasts that were indented with a micrometer-sized bead attached to an AFM cantilever at forces from 30–600 pN found that at such small deformations, the elastic modulus of 100 Pa is largely determined by the presence of the actin cortex.
Elasticity measurement of living cells with an atomic force microscope: data acquisition and processing
TLDR
An implementation of the Hertz model is developed, which simplifies the data processing of elasticity measurement and shows that indenting cells with sharp tips results in higher Young’s moduli than using colloidal probes.
A hybrid high-speed atomic force-optical microscope for visualizing single membrane proteins on eukaryotic cells.
TLDR
This development advances high-speed atomic force microscopy from molecular to cell biology to analyse cellular processes at the membrane such as signalling, infection, transport and diffusion.
...
1
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