High-speed atomic force microscopy of dental enamel dissolution in citric acid.

  title={High-speed atomic force microscopy of dental enamel dissolution in citric acid.},
  author={Alice L. B. Pyne and W. Marks and Loren M Picco and Peter G Dunton and A. Ul{\vc}inas and Michele E Barbour and Si{\^a}n B Jones and J. Gimzewski and Mervyn J Miles},
  journal={Archives of histology and cytology},
  volume={72 4-5},
High-speed atomic force microscopy (HS AFM) in 'contact' mode was used to image at video rate the surfaces of both calcium hydroxyapatite samples, often used as artificial dental enamel in such experiments, and polished actual bovine dental enamel in both neutral and acidic aqueous environments. The image in each frame of the video of the sample was a few micrometers square, and the high-speed scan window was panned across the sample in real time to examine larger areas. Conventional AFM images… Expand
Nano and micro scale analysis of dentin with in vitro and high speed atomic force microscopy
Atomic force microscopy (AFM) has proven useful in the investigation of porous surfaces due to its nanoscale spatial resolution, micron scale range, compatibility with nonconducting materials, andExpand
Combinatorial localized dissolution analysis: Application to acid-induced dissolution of dental enamel and the effect of surface treatments.
A finite element method (FEM) simulation of SECCM mass transport and interfacial reactivity, allows the intrinsic rate constant for acid-induced dissolution to be quantitatively determined and exhibits the greatest protection to the enamel surface, but the effect is again transient. Expand
Measurement of the efficacy of calcium silicate for the protection and repair of dental enamel.
Calcium silicate is a material that has potential for a new approach to the repair of demineralised enamel and the protection of enamel from acid attacks, leading to significant dental hard tissue benefits. Expand
Quantitative crystal investigations : surface dissolution and nucleation processes.
The aim of this thesis is twofold with both elements related to industrially relevant crystal systems and processes. The first element utilized a combined scanning electrochemical cell microscopeExpand
High-speed atomic force microscopy for materials science
Since its inception in 1986, the field of atomic force microscopy (AFM) has enabled surface analysis and characterisation with unparalleled resolution in a wide variety of environments. However, theExpand
Bearing in mind that in the field of jaw orthopedics and related scientific fields, there are no scientific studies that use the most modern technology based on the Atomic Force Microscopy (AFM) toExpand
A study of dynamic nanoscale corrosion initiation events using HS-AFM.
Within this study HS-AFM was combined with SEM and FIB milling to produce a multifaceted picture of localised corrosion events occurring on thermally sensitised AISI 304 stainless steel in an aqueous solution of 1% sodium chloride (NaCl). Expand
Observation of stress corrosion cracking using real-time in situ high-speed atomic force microscopy and correlative techniques
Contact-mode high-speed atomic force microscopy (HS-AFM) has been utilised to measure in situ stress corrosion cracking (SCC) with nanometre resolution on AISI Type 304 stainless steel in anExpand
Evaluation of Surface Roughness Characteristics Using Atomic Force Microscopy and Inspection of Microhardness Following Resin Infiltration with Icon®
The present in vitro study shows that the resin infiltration technique results in increased microhardness of demineralized enamel, however, it was observed that the infiltrant material creates a significantly rougher surface compared to healthy, untreated enamel. Expand
Examining the enamel surface with the Atomic Force Microscopy (AFM) enables more precise registering and defining of the changes of enamel surface structure and of microhardness. This method can beExpand


The Early Stages of Native Enamel Dissolution Studied with Atomic Force Microscopy.
  • Finke, Jandt, Parker
  • Chemistry, Medicine
  • Journal of colloid and interface science
  • 2000
This study showed that the maximum material loss occurred at the aprismatic parts of the enamel close to the perikymata, demonstrating that the AFM is a suitable tool for measuring early stages of enamel demineralization. Expand
A new model for nanoscale enamel dissolution.
CC demineralization of enamel in acidic medium follows this new model that can be used to mimic carious lesion formation, and nanosized enamel crystallites exhibit a remarkable degree of self-preservation in the fluctuating physiological milieu. Expand
Breaking the speed limit with atomic force microscopy
High-speed atomic force microscopy (AFM) is important for following processes that occur on sub-second timescales for studies both in biology and materials science, and also for the ability toExpand
A high-speed atomic force microscope for studying biological macromolecules
The atomic force microscope (AFM) is a powerful tool for imaging individual biological molecules attached to a substrate and placed in aqueous solution. At present, however, it is limited by theExpand
High-speed AFM and nano-visualization of biomolecular processes
This article compares high-speed AFM with its competitor (single-molecule fluorescence microscopy) on various aspects and then describes high- Speed AFM instrumentation and imaging studies on biomolecular processes. Expand
A High-Speed Atomic Force Microscope for Studying Biological Macromolecules in Action
The atomic force microscope (AFM) is a powerful tool for imaging biological molecules on a substrate, in solution. However, there is no effective time axis with AFM; commercially available AFMsExpand
Real-time nanofabrication with high-speed atomic force microscopy.
The application of a previously described high-speed atomic force microscope for nanofabrication concerns the modification of a silicon surface by locally oxidizing the region in the vicinity of the AFM tip. Expand
Components for high speed atomic force microscopy.
A new scanner principle is developed, based on stack piezos, which allows the construction of a scanner with 15 microm scan range while retaining high resonance frequencies (>10 kHz) and to drive the AFM at high scan speeds and record the height and error signal. Expand
High-Speed Atomic Force Microscopy for Studying the Dynamic Behavior of Protein Molecules at Work
In the quest for the mechanism of protein functions, various key techniques and instruments have been developed. This is an era when scrutinizing a certain protein from various angles is becomingExpand
Short cantilevers for atomic force microscopy
We have designed and tested a family of silicon nitride cantilevers ranging in length from 23 to 203 μm. For each, we measured the frequency spectrum of thermal motion in air and water. SpringExpand