High spatial resolution studies of muscarinic neuroeffector junctions in mouse isolated vas deferens

@article{CuprianBeltechi2009HighSR,
  title={High spatial resolution studies of muscarinic neuroeffector junctions in mouse isolated vas deferens},
  author={Alina M. Cuprian-Beltechi and Pravesh Solanki and Noriyoshi Teramoto and Thomas C. Cunnane},
  journal={Neuroscience},
  year={2009},
  volume={162},
  pages={1366-1376}
}
It is acknowledged that neurotransmission in the mouse vas deferens is predominantly mediated by ATP and noradrenaline (NA) released from sympathetic nerves while cholinergic transmission in the rodent vas deferens is often overlooked despite early literature. Recently we have characterized a cholinergic component of neurogenic contraction of mouse isolated vas deferens. In the present paper, by confocal imaging of Ca(2+) dynamics we detected acetylcholine (ACh) action at muscarinic cholinergic… 
Dynamic monitoring of NET activity in mature murine sympathetic terminals using a fluorescent substrate
TLDR
A fluorescence approach for monitoring norepinephrine transporter (NET) transport rate in mature sympathetic terminals is validated and how prejunctional muscarinic receptors affect NET rate is determined.

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TLDR
It has been demonstrated unequivocally that the mouse vas deferens is innervated by functional cholinergic nerves, whose action is terminated by cholinesterase.
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TLDR
Experimental data show that there is a functional cholinergic innervation in the guinea-pig vas deferens whose action is limited by acetylcholinesterase, blocked by cyclopentolate and mediated through M3 receptors.
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TLDR
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TLDR
It is suggested that ATP released from sympathetic varicosities activates the initial, transient, contraction and the activator Ca2+ is derived largely from jCaTs, and sympathetically released noradrenaline activates the later, major contraction through mechanisms involving α1‐adrenoceptors and which are associated with propagating Ca2- waves.
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TLDR
The results suggest that muscarinic receptors are involved in the postsynaptic potentiation of the neurogenic twitch contraction in the mouse vas deferens.
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TLDR
Confocal microscopy of fluo-4 fluorescence in pressurized rat mesenteric small arteries subjected to low-frequency electrical field stimulation revealed Ca2+ transients in perivascular nerves and novel, spatially localized Ca2- entering smooth muscle cells through P2X receptors activated by ATP released from sympathetic nerves, which are called “junctional Ca2 + transients” or jCaTs.
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TLDR
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TLDR
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TLDR
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TLDR
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