High spatial resolution studies of muscarinic neuroeffector junctions in mouse isolated vas deferens

  title={High spatial resolution studies of muscarinic neuroeffector junctions in mouse isolated vas deferens},
  author={Alina M. Cuprian-Beltechi and Pravesh Solanki and Noriyoshi Teramoto and Thomas C. Cunnane},
It is acknowledged that neurotransmission in the mouse vas deferens is predominantly mediated by ATP and noradrenaline (NA) released from sympathetic nerves while cholinergic transmission in the rodent vas deferens is often overlooked despite early literature. Recently we have characterized a cholinergic component of neurogenic contraction of mouse isolated vas deferens. In the present paper, by confocal imaging of Ca(2+) dynamics we detected acetylcholine (ACh) action at muscarinic cholinergic… 
Dynamic monitoring of NET activity in mature murine sympathetic terminals using a fluorescent substrate
A fluorescence approach for monitoring norepinephrine transporter (NET) transport rate in mature sympathetic terminals is validated and how prejunctional muscarinic receptors affect NET rate is determined.


Cholinergic innervation of the mouse isolated vas deferens
It has been demonstrated unequivocally that the mouse vas deferens is innervated by functional cholinergic nerves, whose action is terminated by cholinesterase.
Cholinergic innervation of the guinea-pig isolated vas deferens
Experimental data show that there is a functional cholinergic innervation in the guinea-pig vas deferens whose action is limited by acetylcholinesterase, blocked by cyclopentolate and mediated through M3 receptors.
Cholinergic receptors in the human vas deferens.
The antagonism exerted by PZ on the ACh-induced contractile response of the HVD, suggests that ACh probably acts at a presynaptic level stimulating the release of NE from an adrenergic neuron.
Purinergic and Adrenergic Ca2+ Transients during Neurogenic Contractions of Rat Mesenteric Small Arteries
It is suggested that ATP released from sympathetic varicosities activates the initial, transient, contraction and the activator Ca2+ is derived largely from jCaTs, and sympathetically released noradrenaline activates the later, major contraction through mechanisms involving α1‐adrenoceptors and which are associated with propagating Ca2- waves.
Involvement of the muscarinic receptors in the postsynaptic potentiation of neurogenic twitch contraction in the mouse vas deferens.
The results suggest that muscarinic receptors are involved in the postsynaptic potentiation of the neurogenic twitch contraction in the mouse vas deferens.
Evoked and Spontaneous Purinergic Junctional Ca2+ Transients (jCaTs) in Rat Small Arteries
Confocal microscopy of fluo-4 fluorescence in pressurized rat mesenteric small arteries subjected to low-frequency electrical field stimulation revealed Ca2+ transients in perivascular nerves and novel, spatially localized Ca2- entering smooth muscle cells through P2X receptors activated by ATP released from sympathetic nerves, which are called “junctional Ca2 + transients” or jCaTs.
Intermittent ATP release from nerve terminals elicits focal smooth muscle Ca2+ transients in mouse vas deferens
Monitoring of NCTs now allows transmitter release to be detected simultaneously from each functional varicosity on an identified nerve terminal branch on an impulse‐to‐impulse basis.
Heterogeneity of presynaptic muscarinic receptors mediating inhibition of sympathetic transmitter release: a study with M2‐ and M4‐receptor‐deficient mice
The results clearly indicate that the release‐inhibiting muscarinic receptors on postganglionic sympathetic axons in mouse atria, bladder and vas deferens represent mixtures of M2‐ and non‐M2‐receptors.
Pathways involved in muscarinic M1 and M2 receptor stimulation in rabbit vas deferens.
  • M. Eltze
  • Chemistry, Medicine
    European journal of pharmacology
  • 1994
Findings are consistent with the hypothesis that, in rabbit vas deferens, inhibitory muscarinic M1 receptors stimulate LiCl-sensitive phosphatidylinositol turnover (IP3 pathway) involving protein kinase C, whilst excitatory mus carinic M2 receptors are coupled to inhibition of adenylate cyclase, resulting in reduced levels of cyclic AMP.
Potentiation of sympathetic neuromuscular transmission mediated by muscarinic receptors in guinea pig isolated vas deferens
In guinea-pig isolated vasa deferentia, purinergic neurogenic contractions and responses to applied adenosine 5′-triphosphate (ATP) were potentiated by carbachol; responses to adrenergic transmission and applied noradrenaline were not, suggesting the presence of a non-purinergic, non-adrenergic component.