High sequence specificity of micrococcal nuclease.

  title={High sequence specificity of micrococcal nuclease.},
  author={Colin Dingwall and G. P. Lomonossoff and Ronald A. Laskey},
  journal={Nucleic acids research},
  volume={9 12},
The substrate specificity of micrococcal nuclease (EC has been studied. The enzyme recognises features of nucleotide composition, nucleotide sequence and tertiary structure of DNA. Kinetic analysis indicates that the rate of cleavage is 30 times greater at the 5' side of A or T than at G or C. Digestion of end-labelled linear DNA molecules of known sequence revealed that only a limited number of sites are cut, generating a highly specific pattern of fragments. The frequency of… CONTINUE READING