High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol.

@article{Andeer2012HighsensitivitySP,
  title={High-sensitivity stable-isotope probing by a quantitative terminal restriction fragment length polymorphism protocol.},
  author={Peter F Andeer and Stuart E Strand and David A. Stahl},
  journal={Applied and environmental microbiology},
  year={2012},
  volume={78 1},
  pages={
          163-9
        }
}
Stable-isotope probing (SIP) has proved a valuable cultivation-independent tool for linking specific microbial populations to selected functions in various natural and engineered systems. However, application of SIP to microbial populations with relatively minor buoyant density increases, such as populations that utilize compounds as a nitrogen source, results in reduced resolution of labeled populations. We therefore developed a tandem quantitative PCR (qPCR)-TRFLP (terminal restriction… CONTINUE READING
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