High sensitivity of fetal DNA in plasma compared to serum and nucleated cells using unnested PCR in maternal blood.

@article{HoufflinDebarge2000HighSO,
  title={High sensitivity of fetal DNA in plasma compared to serum and nucleated cells using unnested PCR in maternal blood.},
  author={V{\'e}ronique Houfflin-Debarge and Helen O'Donnell and Timothy Graeme Overton and Phillip R. Bennett and Nicholas M Fisk},
  journal={Fetal diagnosis and therapy},
  year={2000},
  volume={15 2},
  pages={102-7}
}
DNA analysis of blood is conventionally performed on cells - plasma and serum are discarded. Free DNA has been demonstrated in serum in cancer and autoimmune disorders and in pregnancy. We investigated possible noninvasive prenatal diagnosis using fetal DNA from maternal plasma and serum in pregnancy. Fetal gender was determined by PCR on DNA from maternal venous blood, serum and plasma of 65 women by boiling with or without phenol/chloroform extraction. When sensitivities were compared for… CONTINUE READING

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