High sensitivity enzyme-linked immunosorbent assay (ELISA) method for measuring protein carbonyl in samples with low amounts of protein.

@article{Alamdari2005HighSE,
  title={High sensitivity enzyme-linked immunosorbent assay (ELISA) method for measuring protein carbonyl in samples with low amounts of protein.},
  author={Daryoush Hamidi Alamdari and Elena Kostidou and Konstantinos Paletas and Maria Sarigianni and Anastasios G P Konstas and Alexia Karapiperidou and George Koliakos},
  journal={Free radical biology & medicine},
  year={2005},
  volume={39 10},
  pages={1362-7}
}
The oxidative modification of proteins has been shown to play a major role in a number of pathological processes. One such modification is the addition of the carbonyl groups to the amino acid residue in proteins. For the measurement of the carbonyl groups in low concentration protein samples, we have modified the ELISA (enzyme-linked immunosorbent assay) method that was developed by Buss et al. [Buss, I. H; Chan, T. P.; Sluis, K. B.; Domigan, N. M.; Winterbourn, C. C. Protein carbonyl… CONTINUE READING
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