High sensitivity detection of cancer in vivo using a dual-controlled activation fluorescent imaging probe based on H-dimer formation and pH activation.

@article{Ogawa2010HighSD,
  title={High sensitivity detection of cancer in vivo using a dual-controlled activation fluorescent imaging probe based on H-dimer formation and pH activation.},
  author={Mikako Ogawa and N. Kosaka and Celeste Aida S Regino and Makoto Mitsunaga and Peter L. Choyke and Hisataka Kobayashi},
  journal={Molecular bioSystems},
  year={2010},
  volume={6 5},
  pages={888-93}
}
The key to improving the sensitivity of in vivo molecular imaging is to increase the target-to-background signal ratio (TBR). Optical imaging has a distinct advantage over other molecular imaging methods in that the fluorescent signal can be activated at the target thus reducing background signal. Previously, we found that H-dimer formation quenches fluorescence of xanthene fluorophores, and among these, TAMRA had the highest quenching ratio. Another approach to lowering background signal is to… CONTINUE READING