High resolution two-dimensional electrophoresis of proteins.

@article{OFarrell1975HighRT,
  title={High resolution two-dimensional electrophoresis of proteins.},
  author={P. O'Farrell},
  journal={The Journal of biological chemistry},
  year={1975},
  volume={250 10},
  pages={
          4007-21
        }
}
  • P. O'Farrell
  • Published 1975
  • Medicine, Chemistry
  • The Journal of biological chemistry
A technique has been developed for the separation of proteins by two-dimensional polyacrylamide gel electrophoresis. Due to its resolution and sensitivity, this technique is a powerful tool for the analysis and detection of proteins from complex biological sources. Proteins are separated according to isoelectric point by isoelectric focusing in the first dimension, and according to molecular weight by sodium dodecyl sulfate electrophoresis in the second dimension. Since these two parameters are… Expand
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References

SHOWING 1-10 OF 15 REFERENCES
885-888 ' ~ ' 36
  • Proc. Natl. Acad. Sci. U. S. A
  • 1974
Biochemistry 12 , 3273 - Ann
  • 1973
J. Biol. Chem
  • J. Biol. Chem
  • 1973
J. Biol. Chem. J. Biol. Chem
  • J. Biol. Chem. J. Biol. Chem
  • 1973
Anal. Biochem. Ann. N. Y. Acad. Sci
  • Anal. Biochem. Ann. N. Y. Acad. Sci
  • 1971
Anal. Biochem. Biochim. Biophys. Acta Proc. Natl. Acad. Sci. U. S. A. Anal. Biochem
  • Anal. Biochem. Biochim. Biophys. Acta Proc. Natl. Acad. Sci. U. S. A. Anal. Biochem
  • 1970
Biochem. Biophys.' Res. born&n. ROBINSON. A. B. 0974) Proc. Natl. Acad. Sci. U. S. A. Dev. Biol
  • Biochem. Biophys.' Res. born&n. ROBINSON. A. B. 0974) Proc. Natl. Acad. Sci. U. S. A. Dev. Biol
  • 1970
A. 68; 2315-2320
  • Proc. Natl. Acad. Sci. U
  • 1968
SALSER. W., AND GEIDUSCHEK
  • Proc. Natl. Acad. Sci. U
  • 1967
Proc. Natl. Acad. Sci. U. Proc. Natl. Acad. Sci. U
  • Proc. Natl. Acad. Sci. U. Proc. Natl. Acad. Sci. U
  • 1966
...
1
2
...