High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy

@article{Verveer2007HighresolutionTI,
  title={High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy},
  author={Peter J. Verveer and Jim Swoger and Francesco Pampaloni and Klaus Greger and Marco Marcello and Ernst H. K. Stelzer},
  journal={Nature Methods},
  year={2007},
  volume={4},
  pages={311-313}
}
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of… 

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