High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy

  title={High-resolution three-dimensional imaging of large specimens with light sheet–based microscopy},
  author={Peter J. Verveer and Jim Swoger and Francesco Pampaloni and Klaus Greger and Marco Marcello and Ernst H. K. Stelzer},
  journal={Nature Methods},
We report that single (or selective) plane illumination microscopy (SPIM), combined with a new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding that of confocal fluorescence microscopy in large samples. We demonstrate this by imaging large living multicellular specimens obtained in a three-dimensional cell culture. The ability to rapidly image large samples at high resolution with minimal photodamage provides new opportunities especially for the study of… 

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  • S. Hell
  • Physics
    Nature Biotechnology
  • 2003
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