High resolution thermal denaturation analyses of small sequenced DNA restriction fragments containing Escherichia coli lactose genetic control loci.

@article{Hardies1979HighRT,
  title={High resolution thermal denaturation analyses of small sequenced DNA restriction fragments containing Escherichia coli lactose genetic control loci.},
  author={S. C. Hardies and Wolfgang Hillen and T. C. Goodman and R. D. Wells},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 20},
  pages={10128-34}
}
Differential melting curves are reported for four DNA restriction fragments (789, 301, 203, and 95 base pairs in length) spanning the lactose control region. All but the smallest melt with two or more subtransitions. Maps are proposed which identify the positions of regions of different thermal stability in the sequences. The sizes of regions comprising subtransitions range from 60 to 200 base pairs. An analysis is made of the cooperativity exhibited between regions in the sequence. The effect… CONTINUE READING