High resolution scanning electron microscopy of plant chromosomes

  title={High resolution scanning electron microscopy of plant chromosomes},
  author={Gerhard Wanner and Helmut Formanek and R. Martin and Reinhold G. Herrmann},
A preparation technique for high resolution field emission scanning electron microscopy of plant chromosomes is described. [] Key Result With this technique it was possible to document for the first time at high resolution structures such as the centromeric region, the spindle apparatus and the spindle fibre attachment region. The smallest unit of the DNA packed that can be resolved is a “beads on a string”-like structure of the nucleofilament (10–15 nm fibre).
Efficient preparation of plant chromosomes for high-resolution scanning electron microscopy
A highly reproducible technique to prepare plant chromosomes for high-resolution field emission scanning electron microscopy is presented. The procedure allows the production of relatively high
Globular and Fibrous Structure in Barley Chromosomes Revealed by High-Resolution Scanning Electron Microscopy
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High-resolution scanning electron microscopy of human metaphase chromosomes.
Human metaphase chromosomes, prepared for light microscopy were examined by scanning electron microscopy. Use of an osmium impregnation technique eliminated the need for sputter-coating and allowed
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The observations made on prophase nuclei, the intact mitotic apparatus at metaphase and anaphase, and the phragmoplast at telophase agreed well with what is known about plant mitosis and cytokinesis.
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Transmission electron microscopy is useful for special purposes rather than for routine examination, because of its tedious nature and in spite of a slight gain in the clarity of the image no additional features were revealed.
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